Sensitization of locomotor activity to AMPH was observed during conditioning and this effect was blocked in groups given IWP-2 prior to AMPH. Intra-NAc IWP-2 (0.05, 0.5, 1.0 but not 0.001 μg/0.5 μl/side) during test following conditioning with AMPH blocked expression but at a higher dose than was need to block acquisition. I hypothesized that inhibition of Wnt with intra-NAc microinjections of the Wnt palmitoylation inhibitor IWP-2 will dose-dependently block the acquisition and expression of amphetamine (AMPH)-induced CPP in rats. The present study assessed the role of Wnt signalling in DA-mediated incentive learning using the conditioned place preference (CPP) paradigm. Functional interactions between Wnt and dopamine (DA) signalling has been demonstrated using in vitro and tissue physiology approaches, however there remains a lack of in vivo research into the involvement of Wnt in DA-mediated learning in behaving animals. Recently, Wnt signalling has been implicated in synaptic plasticity and learning and memory. The mechanisms by which dopaminergic neurotransmission in the nucleus accumbens (NAc) is involved in incentive learning produced by rewarding stimuli remain unclear.
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